Evaluation of a lysis direct plating method for pediatric blood cultures.

Comparison of blood culture methods for recovery of Legionella pneumophila from the blood of guinea pigs with experimental infection.

Blood was cultured from guinea pigs with experimental Legionella pneumophila serogroup 1 pneumonia, using four different methods. A 0.03-ml amount was spread onto each of several plates of buffered charcoal-yeast extract supplemented with alpha-ketoglutarate (BCYE) (direct plate); 1.5 ml each was inoculated into a BCYE agar-yeast extract broth bottle (biphasic), a pediatric Isolator tube (E. I....

Recovery of Gardnerella vaginalis from blood by the quantitative direct plating method.

This report describes a case of Gardnerella vaginalis sepsis in an obstetric patient whose blood cultures were negative by the conventional BACTEC system but positive by the quantitative direct plating method, which involves the direct inoculation of blood samples on chocolate agar and blood agar plates.

Diagnosis of disseminated mycobacterial infection: testing a simple and inexpensive method for use in developing countries.

With the development of the acquired immunodeficiency syndrome (AIDS) epidemic, the isolation of mycobacteria from blood has become a common problem for clinical laboratories. In this study two methods were used for the recovery of mycobacteria from blood specimens obtained from AIDS patients: (1) direct inoculation of a biphasic medium, and (2) a non-commercial lysis-centrifugation method. A t...

Clinical comparison of the Isolator 1.5 microbial tube and the BACTEC radiometric system for detection of bacteremia in children.

The Isolator 1.5 microbial tube (E. I. du Pont de Nemours & Co., Inc., Wilmington , Del.) was compared with the BACTEC radiometric detection system (Johnston Laboratories, Inc., Cockeysville, Md.) for the detection of bacteremia in children. The Isolator 1.5 is a blood culture system designed for small volumes of blood (0.5 to 1.5 ml). The method involves lysis of the cells of the patient and t...

Lysis Centrifugation Method for the Direct Identification of Positive Blood Cultures Using MALDI-TOF MS